Used primarily as a research tool in biochemistry and cell biology to inhibit mitochondrial electron transport. It blocks complex III in the mitochondrial respiratory chain, making it valuable for studying cellular respiration, reactive oxygen species production, and apoptosis. Commonly applied in studies involving metabolic pathways and mitochondrial function in isolated cells or tissues.

Also used in fisheries management as a piscicide to control unwanted fish populations due to its high toxicity in aquatic environments.