FLI-06 is primarily utilized in biochemical and pharmacological research due to its ability to inhibit protein secretion. It is particularly valuable in studies focusing on the Golgi apparatus, as it disrupts the function of this organelle, providing insights into protein trafficking and processing mechanisms. Researchers use FLI-06 to investigate cellular pathways and the role of secretory processes in various physiological and pathological conditions. Its application extends to cancer research, where it helps in understanding the role of protein secretion in tumor growth and metastasis. Additionally, FLI-06 is employed in drug discovery to screen for compounds that can modulate secretory pathways, offering potential therapeutic targets for diseases related to protein misfolding or mislocalization.