Used as an active pharmaceutical intermediate in the synthesis of protease inhibitors, particularly in the development of certain antiviral drugs. Its chiral structure makes it valuable for constructing stereoselective peptide-like bonds in complex molecules. Commonly employed in research settings for modifying amino acid derivatives to enhance reactivity in coupling reactions. Also utilized in the preparation of enzyme substrates for biochemical assays, especially those involving esterolytic or proteolytic activity. Its nitrophenyl group allows for easy monitoring of reaction progress through colorimetric detection.