Used in the synthesis of antifolate compounds that inhibit enzymes involved in nucleotide biosynthesis, particularly targeting cancer cells. It serves as a key intermediate in developing pharmaceuticals for oncology, especially in drugs that interfere with folate metabolism to suppress tumor growth. Its structure allows conjugation to targeting moieties for improved delivery in receptor-mediated therapies. Also investigated for use in antibody-drug conjugates (ADCs) due to its potent biological activity and ability to be functionalized.