The Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit is widely used in research to measure PEPCK enzyme activity in biological samples. This kit is essential for studying gluconeogenesis, a critical metabolic pathway that converts non-carbohydrate substrates into glucose. By quantifying PEPCK activity, researchers can investigate metabolic disorders, such as diabetes, and understand the regulation of glucose production in the liver and kidneys. It is also applied in studies related to energy metabolism, insulin resistance, and the effects of nutritional or hormonal changes on metabolic pathways. Additionally, the kit is utilized in drug discovery to evaluate compounds that modulate PEPCK activity, offering potential therapeutic targets for metabolic diseases. Its simplicity and accuracy make it a valuable tool in both basic and applied biomedical research.