Phosphofructokinase (PFK) Activity Assay Kit is widely used in biochemical research to measure the activity of the enzyme phosphofructokinase, which plays a critical role in glycolysis. This kit is essential for studying cellular metabolism, particularly in understanding how cells regulate energy production. It is commonly applied in research related to metabolic disorders, cancer metabolism, and diabetes, as PFK activity is often altered in these conditions. Additionally, the kit is used in drug discovery to screen for compounds that modulate PFK activity, potentially leading to new therapeutic agents. It is also utilized in educational settings to teach students about enzyme kinetics and metabolic pathways. The assay is straightforward, making it accessible for both advanced research and teaching laboratories.