The Sorbitol Dehydrogenase (SDH) Activity Assay Kit is primarily used to measure the activity of SDH in various biological samples. This enzyme plays a critical role in the polyol pathway, where it catalyzes the conversion of sorbitol to fructose. The kit is widely applied in research focused on metabolic disorders, particularly diabetes, as the polyol pathway is often implicated in diabetic complications such as neuropathy, retinopathy, and nephropathy. By quantifying SDH activity, researchers can assess the metabolic flux through this pathway and evaluate the impact of therapeutic interventions. Additionally, the kit is utilized in toxicology studies to investigate liver function, as SDH is a liver-specific enzyme and its activity levels can serve as a biomarker for hepatic injury or disease. The assay is also employed in biochemical and clinical studies to understand enzyme kinetics and regulation in different physiological and pathological conditions.