Tris-MES-SDS running buffer is widely used in polyacrylamide gel electrophoresis (PAGE) for the separation of proteins based on their molecular weight. It is particularly effective in SDS-PAGE, where proteins are denatured and coated with SDS, ensuring they migrate through the gel according to size. This buffer system is ideal for resolving small to medium-sized proteins, typically in the range of 1 to 100 kDa, with high resolution and sharp bands. It is commonly employed in molecular biology and biochemistry labs for protein analysis, quality control, and research applications. The buffer's compatibility with downstream techniques, such as Western blotting, makes it a versatile choice for protein characterization and detection.