Azidamfenicol is primarily used as an analytical reference standard in pharmaceutical research and quality control. It serves as a derivative for studying the metabolism and degradation products of chloramphenicol, an antibiotic. Due to its structural similarity, it is employed in the development and validation of analytical methods such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) to detect and quantify chloramphenicol residues in food, biological samples, and environmental matrices. It is not used therapeutically but plays a critical role in ensuring the accuracy of tests aimed at monitoring antibiotic contamination, especially in dairy, honey, and seafood products to comply with food safety regulations.