6-Amino-5-azacytidine is primarily used in research and development within the field of epigenetics. It acts as a DNA methyltransferase inhibitor, making it valuable for studying DNA methylation processes and their role in gene expression. This compound is often employed in experimental studies to investigate cellular differentiation, cancer biology, and the reprogramming of stem cells. By altering methylation patterns, it helps researchers understand how epigenetic changes influence cellular behavior and disease progression. Additionally, it has potential therapeutic applications in targeting diseases linked to abnormal DNA methylation, such as certain cancers and genetic disorders. Its role in modulating gene expression makes it a critical tool in advancing epigenetic therapies.