H-Ala-Glu-OH is primarily used in biochemical research as a dipeptide model to study peptide interactions, enzymatic reactions, and protein folding mechanisms. It serves as a substrate for investigating the activity of proteases and peptidases, aiding in understanding enzyme specificity and kinetics. Additionally, this compound is utilized in the development of synthetic peptides for therapeutic purposes, particularly in designing drug candidates targeting specific biological pathways. Its structure allows for exploration in peptide-based drug delivery systems, where stability and bioavailability are critical. In nutritional science, it may be studied for its potential role in amino acid metabolism and absorption.