Z-Val-Phe-OH is primarily used in peptide synthesis as a building block for creating more complex peptide structures. It is particularly valuable in the production of custom peptides for research purposes, enabling scientists to study peptide interactions, functions, and properties. This compound is also employed in the development of peptide-based drugs, where it contributes to the design of therapeutic agents targeting specific biological pathways. Additionally, Z-Val-Phe-OH is utilized in biochemical assays to investigate enzyme-substrate interactions, aiding in the understanding of enzymatic mechanisms. Its role in solid-phase peptide synthesis (SPPS) makes it a key component in the preparation of peptides for use in pharmaceuticals, diagnostics, and biotechnology applications.