N-Acetylglycylleucine is primarily utilized in the field of biochemistry and pharmaceutical research as a peptide derivative. It serves as a valuable intermediate in the synthesis of more complex peptides and proteins, aiding in the study of peptide structure and function. Additionally, it is employed in the development of drug delivery systems, where its properties can enhance the stability and bioavailability of therapeutic compounds. In cosmetic formulations, it is used for its potential skin-conditioning benefits, helping to improve skin texture and hydration. Its role in enzymatic studies also makes it a useful tool for understanding metabolic pathways and enzyme mechanisms.