Diglycerol is widely used in cosmetic and personal care formulations due to its excellent humectant properties, helping to retain moisture in skin and hair products. It enhances the texture and stability of creams and lotions while reducing irritation caused by other ingredients. In the food industry, it serves as a low-energy sweetener and texture modifier in products like candies and foods for sugar control, and its non-cariogenic properties make it suitable for oral care items such as chewing gum and toothpaste.

In pharmaceuticals, it acts as a solvent and stabilizer in drug delivery systems, especially in topical and ophthalmic preparations, and as a precursor in drug synthesis or for controlled release. Ongoing research explores its use in targeted delivery systems like microemulsions and liposomes. Its low toxicity and biocompatibility make it suitable for medical devices and oral care products.

Additionally, diglycerol serves as a building block in the synthesis of surfactants and polyesters for industrial applications, including biodegradable polymers and functional fluids.