Iodoacetamide is widely used in biochemical research as an alkylating agent to modify cysteine residues in proteins, effectively inhibiting enzyme activity by blocking active sites. This property makes it valuable in proteomics for preventing disulfide bond formation during protein sample preparation, ensuring accurate analysis. Additionally, it is employed in electrophoresis to alkylate free thiol groups, preventing protein aggregation and improving resolution. In molecular biology, iodoacetamide is utilized to inactivate enzymes like papain and bromelain, ensuring controlled experimental conditions. Its ability to irreversibly inhibit enzymes also aids in studying enzyme mechanisms and protein functions.