Guanidine hydrochloride is widely used in molecular biology for protein denaturation. It helps in breaking down the secondary and tertiary structures of proteins, making it useful in studies involving protein folding and unfolding. In biochemistry, it serves as a chaotropic agent, aiding in the solubilization of proteins and nucleic acids, particularly in extraction and purification processes. It is also employed in the preparation of RNA and DNA, where it helps in the isolation of these molecules by disrupting cell membranes and inactivating nucleases. Additionally, guanidine hydrochloride has been used in the treatment of certain medical conditions, such as myasthenia gravis, where it enhances neuromuscular transmission by increasing acetylcholine release. In industrial settings, it is used in the synthesis of various organic compounds, including pharmaceuticals and dyes, due to its strong basic properties.