α-Ketoglutarate Dehydrogenase, derived from porcine heart, is widely used in biochemical research to study the tricarboxylic acid (TCA) cycle. It plays a critical role in catalyzing the conversion of α-ketoglutarate to succinyl-CoA, a key step in cellular energy production. Researchers utilize this enzyme to investigate metabolic pathways, mitochondrial function, and cellular respiration. It is also employed in enzyme kinetics studies to understand its regulatory mechanisms and interactions with other TCA cycle components. Additionally, it serves as a tool in diagnostic assays to assess metabolic disorders related to the TCA cycle. Its application extends to the development of therapeutic strategies targeting mitochondrial dysfunction and metabolic diseases.