Galloflavin is primarily used as a selective inhibitor of lactate dehydrogenase A (LDHA), an enzyme involved in anaerobic glycolysis. This makes it a valuable tool in cancer research, as many tumor cells rely heavily on LDHA for energy production under hypoxic conditions. By inhibiting LDHA, galloflavin disrupts the metabolic pathway that supports rapid cancer cell proliferation, leading to reduced tumor growth in preclinical models. It is also employed in studies related to cellular metabolism, helping scientists understand the role of LDHA in immune cell function and metabolic reprogramming. Due to its ability to shift cells from glycolytic to oxidative metabolism, galloflavin has been explored in research on metabolic diseases and inflammation. Additionally, galloflavin serves as a reference compound in high-throughput screening assays designed to identify new LDHA inhibitors, supporting drug discovery efforts in oncology and beyond.