This compound is primarily used in biochemical research as a potent and selective inhibitor of caspase enzymes, particularly caspase-1, caspase-3, and caspase-7. It plays a significant role in studying apoptosis, or programmed cell death, by blocking the activity of these caspases, which are key mediators of the process. Researchers utilize it to investigate pathways involved in cell death, inflammation, and neurodegenerative diseases. Its application extends to understanding the mechanisms of diseases like Alzheimer's, Parkinson's, and ischemic injuries, where caspase activity is implicated. Additionally, it is employed in drug discovery and development to screen potential therapeutic agents targeting caspase-related pathways.