Ac-DEVD-CHO is widely used in biochemical research as a potent and selective inhibitor of caspase-3, an enzyme involved in apoptosis, or programmed cell death. Its application is crucial in studying the mechanisms of apoptosis and identifying pathways that regulate cell survival and death. Researchers utilize this compound to inhibit caspase-3 activity in cell cultures and experimental models, allowing them to investigate the role of this enzyme in various diseases, including cancer, neurodegenerative disorders, and ischemic injuries. Additionally, it serves as a valuable tool in drug discovery and development, helping to screen and evaluate potential therapeutic agents targeting apoptosis-related pathways. Its specificity and effectiveness make it a key reagent in understanding cellular processes and developing treatments for apoptosis-related conditions.