Liver Infusion is commonly used in microbiological culture media to support the growth of fastidious microorganisms. It provides a rich source of nutrients including amino acids, peptides, vitamins, and minerals that are essential for the cultivation of anaerobic bacteria and other sensitive organisms. It is particularly valuable in preparing media for the isolation and identification of pathogens in clinical laboratories. Liver infusion is also used in the production of vaccines and in research settings where high-nutrient broths are required for optimal microbial growth. Its biological origin makes it suitable for simulating in vivo conditions in laboratory cultures.