Used in microbiology laboratories to isolate and identify Staphylococcus species, particularly Staphylococcus aureus, from clinical and non-clinical samples. It inhibits the growth of most gram-negative bacteria and other contaminants, allowing selective growth of staphylococci. Often used in food safety testing, clinical diagnostics, and environmental monitoring to detect potential pathogens. Some formulations include indicators that differentiate S. aureus from other Staphylococcus species based on mannitol fermentation or enzyme activity.