5-Methoxy-2H-chromen-2-one is primarily utilized in the synthesis of various organic compounds, particularly in the development of pharmaceuticals and agrochemicals. It serves as a key intermediate in the production of anticoagulant agents, which are critical in preventing blood clots in patients with cardiovascular conditions. Additionally, it is employed in the creation of fluorescent dyes and probes, which are essential tools in biochemical research for tracking and visualizing cellular processes. Its role in organic synthesis also extends to the preparation of fragrances and flavoring agents, contributing to the sensory attributes of consumer products. Furthermore, it is explored in material science for the development of organic light-emitting diodes (OLEDs) and other advanced materials.