Column-layer chromatographic silica gel is widely used in the separation and purification of complex mixtures in both analytical and preparative chemistry. It serves as a stationary phase in column chromatography, enabling the separation of compounds based on their polarity, size, or affinity. This material is particularly valuable in the pharmaceutical industry for isolating active ingredients, in natural product chemistry for purifying plant extracts, and in biochemistry for separating proteins, peptides, and nucleic acids. Its high surface area and tunable pore size make it effective for adsorbing and releasing target molecules during the chromatographic process. Additionally, it is employed in thin-layer chromatography (TLC) for rapid qualitative analysis of mixtures, aiding in the identification of components. Its versatility and efficiency make it a crucial tool in research, quality control, and industrial applications.