Violuric acid monohydrate is primarily used in analytical chemistry as a reagent for the detection and determination of various metal ions. It forms colored complexes with metals like copper, iron, and nickel, making it useful in qualitative and quantitative analysis. Additionally, it serves as a precursor in the synthesis of certain organic compounds, particularly in the development of dyes and pigments. Its role in biochemical research includes studying enzyme inhibition and oxidative processes due to its reactive properties. In industrial applications, it is sometimes employed in the production of specialty chemicals and as a stabilizer in certain formulations.