CY5-YNE is widely used in bioimaging and bioconjugation applications due to its fluorescent properties. It is particularly effective in labeling biomolecules such as proteins, nucleic acids, and antibodies for visualization in fluorescence microscopy and flow cytometry. Its alkyne group allows for efficient click chemistry reactions, enabling precise and stable conjugation with azide-modified molecules. This makes it a valuable tool in studying cellular processes, molecular interactions, and diagnostic assays. Additionally, its near-infrared fluorescence is advantageous for deep-tissue imaging in live animal studies, minimizing background interference.