Cinchonine monohydrochloride hydrate is primarily used in organic chemistry as a chiral resolving agent to separate enantiomers from racemic mixtures, facilitating the synthesis of optically active compounds. It plays a key role in asymmetric catalysis for producing enantiomerically pure substances essential in the pharmaceutical industry. Additionally, it has applications in antimalarial medications due to its ability to inhibit the growth of Plasmodium parasites, and it is used in pharmacological research to study antimalarial efficacy and mechanisms. In research laboratories, it supports studies on stereochemistry, reaction mechanisms, and the synthesis of important pharmaceutical chemicals.