Widely used as a chromogenic substrate in enzymatic assays, particularly for detecting and quantifying beta-N-acetylglucosaminidase activity. The compound releases a yellow-colored product upon enzymatic hydrolysis, enabling spectrophotometric detection at around 400–410 nm. Commonly applied in clinical diagnostics, microbiology, and biochemical research to assess enzyme activity in biological samples such as urine, serum, and cell lysates. Its high specificity and sensitivity make it suitable for both manual and automated assay systems. Also employed in studies related to lysosomal storage disorders and microbial identification.