Column-layer chromatographic silica gel is widely used in the separation and purification of complex mixtures in both analytical and preparative chemistry. It serves as a stationary phase in column chromatography, enabling the separation of compounds based on their polarity, size, and interactions with the silica surface. This material is particularly effective in purifying organic compounds, natural products, and pharmaceuticals, as it provides high resolution and efficiency. In thin-layer chromatography (TLC), it is applied as a coating on plates to quickly analyze and identify components in a mixture. Its versatility and stability make it a preferred choice for isolating target molecules, removing impurities, and analyzing chemical compositions in research and industrial laboratories. Additionally, it is utilized in the purification of proteins, peptides, and other biomolecules in biochemical applications.