Used primarily as a derivatization agent in gas chromatography (GC) and mass spectrometry (MS) for the analysis of compounds containing active hydrogens, such as alcohols, phenols, carboxylic acids, and amines. It effectively silylates these functional groups, enhancing their volatility and thermal stability, which improves detection and separation in analytical procedures.

Commonly applied in environmental testing, pharmaceutical analysis, and metabolomics to analyze complex mixtures. Its ability to derivatize polar compounds makes it valuable for studying metabolic pathways and identifying biomarkers in biological samples. Additionally, it is utilized in the synthesis of organosilicon compounds and as a reagent in organic chemistry for protecting hydroxyl groups during reactions.