Z-DEVD-FMK is widely used in biochemical research as a potent and selective inhibitor of caspase-3, a key enzyme involved in apoptosis (programmed cell death). It is particularly valuable in studies aimed at understanding the mechanisms of cell death and survival. By inhibiting caspase-3 activity, researchers can investigate the role of this enzyme in various cellular processes and disease models, such as neurodegenerative disorders, cancer, and ischemic injuries. Additionally, Z-DEVD-FMK is utilized in vitro to prevent apoptosis in cell cultures, ensuring the survival of cells during experimental procedures. Its application extends to drug discovery, where it helps identify potential therapeutic agents targeting apoptosis pathways.