Desfluoro-ezetimibe is primarily utilized in the research and development of pharmaceuticals, particularly in the study of cholesterol absorption inhibitors. It serves as a key intermediate or reference compound in the synthesis of ezetimibe, a drug widely used to manage hypercholesterolemia by reducing the absorption of cholesterol in the intestine. Researchers use desfluoro-ezetimibe to better understand the structure-activity relationship and to optimize the efficacy of cholesterol-lowering drugs. Additionally, it aids in the development of analytical methods for quality control and pharmacokinetic studies, ensuring the safety and effectiveness of related therapeutic agents.