Used as a stable isotope-labeled tracer in metabolic studies to track glutamate metabolism in cells and tissues. Helps in understanding neurotransmitter cycling in the brain, particularly in neuroscience research involving NMR or mass spectrometry. Applied in clinical studies to investigate amino acid metabolism in diseases like cancer, liver disorders, and neurological conditions. Also employed in labeled protein synthesis studies where incorporation of 13C-glutamate allows precise monitoring of metabolic flux in cell cultures or living organisms.