Used as a stable isotope-labeled tracer in metabolic studies to track glycine metabolism in biological systems. Commonly applied in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) for analyzing metabolic fluxes in cells, tissues, and whole organisms. Helps investigate pathways such as one-carbon metabolism, purine synthesis, and glutathione production. Employed in clinical research to study metabolic disorders, cancer metabolism, and liver function. Also used in the development and validation of quantitative analytical methods due to its isotopic purity.