Used as a stable isotope-labeled tracer in metabolic studies to track glycine metabolism in biological systems. Commonly applied in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) for precise detection and quantification of metabolic pathways, including one-carbon metabolism and glutathione synthesis. Supports research in cancer metabolism, liver function, and amino acid dynamics in vivo. Also employed in the development and validation of analytical methods for biomedical and pharmaceutical applications.