Used as a stable isotope-labeled internal standard in quantitative analytical methods, particularly in mass spectrometry and HPLC, to accurately measure levels of 5-hydroxymethylfurfural (HMF) in food, pharmaceuticals, and biological samples. Its primary application lies in research settings for tracking HMF formation and degradation, studying carbohydrate metabolism, and assessing thermal processing effects in products like honey, fruit juices, and milk-based formulations. The 13C6 label allows for precise differentiation from endogenous HMF, enhancing measurement accuracy and reliability in complex matrices.