Blue Dextran is widely used in biochemical and biophysical research as a marker or tracer due to its high molecular weight and distinctive blue color. It is commonly employed in gel filtration chromatography to determine the void volume of columns, helping to calibrate and assess the performance of the separation process. Additionally, it serves as a useful tool in studying membrane permeability and pore size, as its large size allows researchers to evaluate the exclusion limits of various membranes. In protein purification, Blue Dextran aids in monitoring the elution profile and ensuring proper column packing. Its applications also extend to educational laboratories, where it is used to demonstrate principles of chromatography and molecular exclusion techniques.