L-(-)-Sorbose is primarily used in the production of ascorbic acid (vitamin C), where it serves as a key intermediate in the Reichstein process. This process involves the conversion of L-(-)-Sorbose to 2-keto-L-gulonic acid, which is then transformed into ascorbic acid. Additionally, it finds applications in the food industry as a low-calorie sweetener and in the synthesis of other sugar derivatives. Its role in biochemical research is also notable, particularly in studies involving carbohydrate metabolism and enzyme activity.