D-Glucose 6-phosphate dipotassium salt is widely used in biochemical research and enzymatic studies. It serves as a critical substrate in metabolic pathways, particularly in glycolysis and the pentose phosphate pathway, allowing researchers to study energy production and biosynthetic processes in cells. This compound is essential in assays for enzymes like glucose-6-phosphate dehydrogenase, helping to understand metabolic disorders and enzyme kinetics. Additionally, it is utilized in cell culture media to provide an energy source for growing cells, supporting studies in cellular metabolism and function. Its role in diagnostic kits also aids in measuring enzyme activity in clinical samples, contributing to medical diagnostics and research.