D(+)-Raffinose pentahydrate is widely used in the food industry as a functional sweetener and prebiotic. It is incorporated into various food products, such as dairy items, baked goods, and beverages, to enhance texture, stability, and sweetness without significantly increasing caloric content. Its prebiotic properties promote the growth of beneficial gut bacteria, supporting digestive health.

In the pharmaceutical sector, it serves as an excipient in drug formulations, particularly in stabilizing proteins and vaccines during freeze-drying processes. Its ability to protect biological molecules from degradation makes it valuable in the production of biopharmaceuticals.

Additionally, it is utilized in scientific research as a cryoprotectant for preserving cells and tissues during freezing, ensuring their viability for future use in studies or medical applications. Its non-toxic nature and compatibility with biological systems make it a preferred choice in these fields.