D-Fructose 6-phosphate disodium salt hydrate is widely used in biochemical research and enzymatic studies. It serves as a key intermediate in glycolysis, making it essential for investigating metabolic pathways and energy production in cells. This compound is often utilized in assays to study the activity of enzymes such as phosphofructokinase and fructose bisphosphatase, which play critical roles in carbohydrate metabolism. Additionally, it is employed in the preparation of cell culture media to support the growth of microorganisms and mammalian cells. Its role in metabolic research also extends to studies on diabetes, obesity, and other metabolic disorders, providing insights into glucose utilization and regulation.