L-2-Phosphoglyceric acid disodium salt hydrate is primarily used in biochemical research and enzymatic studies. It serves as a key intermediate in glycolysis, making it valuable for investigating metabolic pathways and enzyme kinetics. This compound is also utilized in the preparation of buffers and reagents for studying phosphoglycerate mutase and other enzymes involved in carbohydrate metabolism. Additionally, it finds application in cell culture studies to understand cellular energy production and metabolic processes. Its role in these areas helps advance knowledge in biochemistry, molecular biology, and related fields.