N-Ethyl-D-glucamine is widely used in the pharmaceutical industry as a chiral building block for the synthesis of various drugs, particularly those requiring stereochemical specificity. It serves as a key intermediate in the production of active pharmaceutical ingredients (APIs) for treatments targeting conditions like diabetes and cardiovascular diseases.

In biochemistry, it is employed as a buffering agent due to its ability to maintain stable pH levels in enzymatic reactions and cell culture media. Its solubility in water and compatibility with biological systems make it a valuable component in research and diagnostic applications.

Additionally, N-Ethyl-D-glucamine is utilized in the formulation of contrast agents for medical imaging, such as MRI, where it enhances the clarity and accuracy of images by improving the solubility and stability of gadolinium-based compounds. Its non-toxic nature and ability to chelate metal ions also make it suitable for use in various industrial processes, including water treatment and metal recovery.