Pseudouridine is widely used in RNA research and therapeutic development due to its ability to enhance the stability and translational efficiency of RNA molecules. It is commonly incorporated into messenger RNA (mRNA) vaccines to reduce immune activation and increase protein expression, improving the efficacy and safety of vaccines. This modification helps the RNA evade detection by the innate immune system, minimizing inflammatory responses while prolonging the lifespan of the mRNA in cells. Pseudouridine is also studied for its role in regulating RNA function and structure, contributing to advancements in gene therapy and RNA-based treatments for diseases including cancer and genetic disorders.