Lichenan is primarily used in scientific research, particularly in studies related to carbohydrate chemistry and enzymology. It serves as a substrate for analyzing the activity of enzymes such as lichenase and β-glucanases, which break down β-1,3 and β-1,4 glycosidic linkages in polysaccharides. Due to its unique structure, lichenan is also employed in the development and calibration of analytical methods like chromatography and spectrometry for polysaccharide characterization. Additionally, it has applications in the food and feed industry for assessing digestibility and fermentability of dietary fibers. Its gel-forming properties are explored in some biotechnological processes, including immobilization of cells or enzymes.