FITC Dextran is widely used as a fluorescent tracer in biological and biomedical research. Its primary application lies in studying vascular permeability and blood flow due to its ability to emit bright green fluorescence when excited by light. Researchers use it to visualize and quantify the leakage of molecules across endothelial barriers in tissues, making it valuable in inflammation and tumor angiogenesis studies. It is also employed in cell biology to track dextran uptake in endocytosis assays and to label and monitor the movement of molecules within cells and tissues. Due to its water solubility and biocompatibility, FITC Dextran is commonly used in microcirculation experiments, such as intravital microscopy, where real-time imaging of capillary function is required. In tissue engineering and drug delivery research, it serves as a model macromolecule to evaluate the diffusion properties of hydrogels and scaffolds. Its fluorescence allows for easy detection and quantification in both in vitro and in vivo systems.