FITC Dextran is widely used as a fluorescent tracer in biological and medical research. Its primary application lies in studying vascular permeability and blood flow due to its ability to remain confined within blood vessels under normal conditions. Researchers use it to visualize and quantify leakage in endothelial barriers, making it valuable in inflammation, tumor angiogenesis, and ischemia studies. It is also employed in cell biology to track endocytosis and intracellular transport, as the dextran component is internalized by cells and can be monitored via fluorescence microscopy. Due to its water solubility and low toxicity, FITC Dextran is ideal for in vivo imaging and real-time monitoring of physiological processes in live animals. In tissue engineering and drug delivery research, it serves as a model macromolecule to assess diffusion through hydrogels and scaffolds. Its fluorescence properties allow for easy detection and quantification in assays involving membrane integrity, barrier function, and microdialysis.