Used as a key intermediate in biochemical research, particularly in studies related to glycolysis and gluconeogenesis pathways. It serves as a substrate in enzyme assays involving phosphofructokinase, fructose-1,6-bisphosphatase, and other metabolic enzymes. Commonly employed in cell-free systems and metabolic engineering to simulate or manipulate carbohydrate metabolism. Also utilized in the development and calibration of diagnostic kits for metabolic disorders. Its dipotassium salt form enhances solubility and stability in aqueous solutions, making it suitable for in vitro applications.