Used in microbiology as a selective agent in culture media to isolate and identify specific bacteria based on their ability to ferment raffinose. Commonly found in diagnostic media for Enterobacteriaceae family, helping differentiate species like Salmonella and Shigella. Also serves as a cryoprotectant in preserving cells and enzymes during freezing. In plant tissue culture, supports growth and acts as an energy source. Additionally, used in research to study carbohydrate metabolism and prebiotic effects in gut microbiota.