Used as a metabolic intermediate in biochemical research, particularly in studies involving glycolysis and gluconeogenesis. It acts as an allosteric activator for phosphofructokinase, aiding in the investigation of enzyme kinetics and metabolic pathway regulation. Commonly employed in cell-free systems and enzyme assays to mimic intracellular conditions and study phosphoglucomutase activity, for which it serves as a cofactor. Also utilized in the development and calibration of diagnostic kits for metabolic disorders. Its potassium salt form enhances solubility and stability in aqueous solutions, making it suitable for in vitro applications.